Reporter

Part:BBa_K3510000:Design

Designed by: team work   Group: iGEM20_Tuebingen   (2020-09-30)


FAST2 reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to obtain compliance with the RFC10-BioBrick assembly standard, the literate sequence was altered by 1bp at position 120 (guanin to adenin), so that the PstI-site was removed


Source

The FAST2-fluorescent Tag and the complementary fluorogens were engineered by the Twinkle Factory, in the original puplication by Tebo et al. (Biochemistry 57, 5648-5653 (2018).), the FAST2-Tag was named iFAST.

References